3,5-Dinitrosalicylic acid (DNS) reagent is widely used in the estimation of reducing sugars. The reagent shows a differential behaviour towards mono- and di-saccharides.

How does DNS stop a reaction?

The enzymatic reaction stops due to the dramatic increase of pH (DNS reagent is approximately pH 12-13), though of course, this would depend on the optimum pH of your enzyme, and if it is optimum in alkaline environments, the reaction would stop once the samples are placed in 100 degrees C for 5 minutes because the …

What does adding the DNS solution do to the catalyzed reaction How does it do this?

how does adding DNS stop the enzymatically catalyzed reaction? … as temp increases, the reaction rate increases by increasing the ph of the reactants. when you boil the enzyme you will denature the enzyme and the reaction will no longer be able to produce glucose which means no ANS would be present.