Kcat is the turnover number — the number of substrate molecule each enzyme site converts to product per unit time. If you know the concentration of enzyme sites, you can fit Kcat instead of Vmax when analyzing a substrate vs.

What is the kcat of a reaction?

It is built in to Prism (starting with Prism 5) in the enzyme kinetics group of equations. … Kcat is turnover number, expresed as number of substrate molecules turned into product per enzyme site per minute. Km and X are both in the same units of substrate concentration.

What does a lower kcat mean?

One way to measure the catalytic efficiency of a given enzyme is to determine the kcat/km ratio. … The greater the ratio, the higher the rate of catalysis is; conversely, the lower the ratio, the slower the catalysis is.