To prepare stock solution A, fill a beaker with 100 mL of water. Add 0.18 g of luminol and 3.0 mL of sodium hydroxide solution (1 M).To prepare stock solution B, fill another beaker with 100 mL of water. Add 1 mL of hydrogen peroxide (3%) and 0.03 g of potassium ferricyanide.

How do you make a luminol solution?

1. To prepare stock solution A, fill a beaker with 100 mL of water. Add 0.18 g of luminol and 3.0 mL of sodium hydroxide solution (1 M).
2. To prepare stock solution B, fill another beaker with 100 mL of water. Add 1 mL of hydrogen peroxide (3%) and 0.03 g of potassium ferricyanide.

How is luminol created?

Luminol is synthesized in a two-step process, beginning with 3-nitrophthalic acid. First, hydrazine (N2H4) is heated with the 3-nitrophthalic acid in a high-boiling solvent such as triethylene glycol and glycerol. An acyl substitution condensation reaction occurs, with loss of water, forming 3-nitrophthalhydrazide.