Periodic acid–Schiff (PAS) is a staining method used to detect polysaccharides such as glycogen, and mucosubstances such as glycoproteins, glycolipids and mucins in tissues. … A suitable basic stain is often used as a counterstain.

What cells would PAS stain?

Germ Cell Neoplasms of the Ovary Periodic acid–Schiff (PAS) stain highlights the abundant glycogen in the tumor cells; the staining will disappear after diastase treatment. By immunohistochemistry, dysgerminoma cells are positive for PLAP (strong, cytoplasmic staining), SALL4, CD117, and OCT4 (Fig. 16.7).

What does PAS stain in fungi?

The PAS stain is a simple stain that demonstrates polysaccharides. Cellulose and chitin, two substances rich in polysaccharides, are found in the cell walls of fungi. The addition of the enzyme diastase (PASd) eliminates the potential of confounding results caused by the presence of glycogen.