Principle: Chelex resin works by preventing DNA degradation from degradative enzymes (DNases) and from potential contaminants that might inhibit downstream analyses. In general, the Chelex resin will trap such contaminants, leaving DNA in solution. … This releases DNA into solution.

What is Chelex and how does it function?

Chelex 100 is a chelating material from Bio-Rad used to purify other compounds via ion exchange. It is noteworthy for its ability to bind transition metal ions. … Chelex protects the sample from DNases that might remain active after the boiling and could subsequently degrade the DNA, rendering it unsuitable for PCR.

What is the function of ammonium acetate in DNA extraction?

In order to remove impurities and concentrate the DNA in solution, we have introduced modifications in the existing DNA isolation protocol using Chelex-100. We used ammonium acetate to precipitate proteins and a sodium acetate- isopropanol mixture to pellet out DNA which was washed with ethanol.