Basically, make 0.2 M cacodylic acid and 0.2 M NaOH. Add 0.2 M NaOH to 0.2 M cacodylic acid to desired pH. Adjust 50 ml of 0.2M cacodylic acid to desired pH with 0.2M NaOH. Dilute to 100 ml with ddH2 or dilute 1:1 with fixative.

How do you make a sodium cacodylate buffer?

Sodium Cacodylate Buffers:

0.1M: Sodium cacodylate 4.28 gm Calcium chloride 25.0 gm 0.2N hydrochloric acid 2.5 ml Dilute to 200 ml with distilled water, pH 7.4 0.2M: Sodium cacodylate 8.56 gm Mix as above, pH to 7.4 with HCL.

What is cacodylate buffer?

Definition: A buffer solution in which the pH (6.4--7.4) is maintained by an cacodylic acid-- sodium cacodylate [Na(CH3)2 AsO2] equilibrium.