As a distinctive feature, confocal microscopy enables the creation of sharp images of the exact plane of focus, without any disturbing fluorescent light from the background or other regions of the specimen. Therefore, structures within thicker objects can be conveniently visualized using confocal microscopy.

When would you use a confocal laser scanning microscopy?

1 Confocal Laser Scanning Microscopy. CLSM has been the workhorse for dynamic imaging of early stages of neural crest induction and migration as it provides high lateral (x–y) and axial (z) resolution.

Why would you use confocal microscopy?

Confocal microscopy provides many advantages over conventional widefield microscopy for life sciences applications. It allows control of depth-of-field and the ability to collect serial optical sections from thick specimens. Confocal microscopy can be used to create 3D images of the structures within cells.