An autoclave is used in medical and laboratory settings to sterilize lab equipment and waste. Autoclave sterilization works by using heat to kill microorganisms such as bacteria and spores. ... It's nontoxic and inexpensive, it kills microbes and spores rapidly, and it quickly heats and penetrates fabrics.

What does autoclaving not destroy?

Moist heat, such as conventional autoclaving will not destroy endotoxin and, by the nature of destroying Gram-negative bacteria, can actually contribute to increasing endotoxin levels through cell lysis.

Can autoclaving be used to sterilize everything?

The autoclave's benefits were soon evident, and it became an essential part of every clinic and hospital. An autoclave is used to sterilize surgical equipment, laboratory instruments, pharmaceutical items, and other materials. It can sterilize solids, liquids, hollows, and instruments of various shapes and sizes.