The In-Gel Tryptic Digestion Kit provides a complete set of reagents for performing approximately 150 digestions on colloidal coomassie- or fluorescent dye-stained protein bands. ... Two key sample preparation steps are the fragmentation of proteins into peptides and subsequent concentration and clean up of peptides.

What is tryptic digestion?

Tryptic Digestion. NCI Thesaurus. Code C68835. A technique for proteolysis of proteins into peptides at lysine or arginine by treatment with the pancreatic enzyme trypsin.

How do you do gel digestion?

The in-gel digestion step primarily comprises the four steps; destaining, reduction and alkylation (R&A) of the cysteines in the protein, proteolytic cleavage of the protein and extraction of the generated peptides.