Type II restriction enzymes also differ from types I and III in that they cleave DNA at specific sites within the recognition site; the others cleave DNA randomly, sometimes hundreds of bases from the recognition sequence.

Do restriction enzymes cut DNA randomly?

Restriction enzymes cut DNA randomly. ... Restriction enzymes recognize specific sequences on DNA and always cuts so that only blunt ends are produced. 3. Restriction enzymes can be used to compare different genomes based on the produced pattern of DNA fragments.

How is the DNA cleaved?

A reaction that severs one of the covalent sugar-phosphate linkages between NUCLEOTIDES that compose the sugar phosphate backbone of DNA. It is catalyzed enzymatically, chemically or by radiation. Cleavage may be exonucleolytic - removing the end nucleotide, or endonucleolytic - splitting the strand in two.