The importance of fixation
The broad objective of tissue fixation is to preserve cells and tissue components in a “life-like state” and to do this in such a way as to allow for the preparation of thin, stained sections.
Why is immediate fixation important?
Immediate fixation is important to preserve the tissue in a lifelike state. Fixation makes the tissue more receptive to dyes by setting up amino acids and charges so staining works properly. Most stains are designed for formalin fixed paraffin embedded (FFPE) tissue.
What is the purpose of fixation in microbiology?
Fixation is process by which the internal & external structures of cells & microorganisms are preserved & fixed. It inactivates enzymes that might disrupt cell morphology & toughens cell structures so that they don't change during staining & observation.
