Affinity chromatography is a method of separating a biomolecule from a mixture, based on a highly specific macromolecular binding interaction between the biomolecule and another substance.

What is the principle of affinity chromatography?

The principle of affinity chromatography is that the stationary phase consists of a support medium (e.g. cellulose beads) on which the substrate (or sometimes a coenzyme) has been bound covalently, in such a way that the reactive groups that are essential for enzyme binding are exposed.

What is affinity in TLC chromatography?

Affinity chromatography. solid (agarose or porous glass beads on to which are immobilized molecules like enzymes and antibodies) liquid. binding affinity of the analyte molecule to the molecule immobilized on the stationary phase.