To cue audio is to determine the desired initial playback point in a piece of recorded music. It is a technique often used in radio broadcasting and DJing. One dictionary definition is to "Set a piece of audio or video equipment in readiness to play (a particular part of the recorded material)."

What are soundtrack cues?

What is a Music Cue? The term Music Cue originates from theatrical productions and relates to the insertion of music as a prompt for a performer or stage hand to carry out an action or deliver a line.

What does it mean to cue in on something?

As a verb, queue means get in line or place in line. Both these homophones are often used with up—cue up meaning prepare [something] to start on cue, and queue up meaning get in line.