Running mid-rolls on your channel helps disable pre-rolls. Subs and Turbo users do not see ads (caveat: a small portion of creators do turn ads on for subs. The vast majority of creators choose not to show ads to subs.

Can you get rid of ads on Twitch?

Streamers can now disable pre-roll ads for incoming viewers by regularly running ad breaks during their stream, allowing viewers to immediately see what's going on. When a streamer runs a 30 second ad break, pre-roll ads will be disabled for the next 10 minutes.

What does subbing on Twitch do?

A Subscription allows a viewer to pay a minimum of $4.99 per month to support your channel, either on a recurring or one-time basis. Subscribers (subs) get access to your emotes and other benefits you can define.