The pre-heating of the elution buffer is essential for maximal recovery with the Monarch^® Genomic DNA Purification Kit. ... Additionally, at room temperature, large gDNA fragments will elute less efficiently, so the eluted gDNA will have a smaller average size.

Why do we use elution buffer?

Elution buffer is used to wash away unbound proteins at first and at a greater concentration it releases the desired protein from the ligand. It is important that the elution buffer works quickly without changing the function or activity of the desired protein.

What is the function of elution buffer in DNA extraction?

Your lysis buffer is used when you resuspend and break open your cells. It needs to have a low imidazole concentration (2-5mM is usual I think) to promote tagged protein binding and suppress non-specific binding. Your elution buffer is used to remove the tagged protein of interest from the column.