Quantification of DNA is a very important step in many procedures where it is necessary to know the amount of DNA that is present when carrying out restriction digests or performing different techniques such as PCR and RAPDs.

Why is it important to quantify nucleic acids?

Nucleic acid quantification is essential to obtain accurate and reliable results in many molecular biology assays such as PCR (polymerase chain reaction) and real-time PCR reactions. Low amounts of template can lead to insufficient amplification and lack of yields, and therefore bear the risk of false negative results.

What is the importance of blank in quantification of DNA?

Before measuring any samples, be sure to 'blank' the spectrophotometer using the solution the DNA is resuspended in, but with no DNA added. 'Blanking' measures the background inherent to the machine and your solvent. If using a NanoDrop to measure your samples, place 1-2µL of mini-prepped DNA onto the pedestal.