The quick answer to the question is most likely yes. It depends a bit on what type of skin they possess. If their skin is fragile then the spiders will almost certainly die. If being sucked through the hose doesn't do the job then the dirt and dust in the vacuum bag will finish off the little creatures via suffocation.

Do spiders survive vacuuming?

So, can spiders survive being vacuumed? Most spiders cannot survive being vacuumed. They can die immediately, die from the trauma of being sucked into the vacuum cleaner, or eventually die from thirst. If the vacuum cleaner has a rotating brush, spiders may lose one or several of their legs.

How does a vacuum kill a spider?

When it comes to spiders and bugs that have a more fragile body without an exoskeleton, they most likely will be killed by the suction. If they make it into the vacuum bag, they will suffocate from the dirt inside. Bugs that do survive the suction and stay alive in the vacuum bag can crawl out.