The enzyme hexokinase requires a magnesium ion as a cofactor. Magnesium ions function to shield the negatively charged groups of ATP and facilitate the reaction with glucose. Coenzymes are also cofactors.

Which cofactor is required by hexokinase?

A hexokinase is an enzyme that phosphorylates hexoses (six-carbon sugars), forming hexose phosphate. ... In most organisms, glucose is the most important substrate for hexokinases, and glucose-6-phosphate is the most important product.

How is hexokinase activated?

Hexokinase activates glycoloysis by phosphorylating glucose. ... Tissues where hexokinase is present use glucose at low blood serum levels. G6P inhibits hexokinase by binding to the N-terminal domain(this is simple feedback inhibition). It competitively inhibits the binding of ATP [8].