We should first recognize that there was not one system of punctuation in use during Classical times. However, all of modern punctuation, including commas, periods, colons, semicolons, etc. ... In the earliest inscriptions, too, you can see a lack of word division or punctuation.

Do they use commas in Latin?

Yes, modern editions of ancient texts and modern texts written in Latin or translated into Latin generally use punctuation, including commas.

When was the comma invented?

Aldus Manutius (also known as Aldo Manuzio) was a 15th-century Italian printer who introduced the comma as we know it, as a way to separate things. The word comma comes from the Greek word koptein, which means "to cut off."