In the first step of glycolysis, the enzyme hexokinase uses ATP to transfer a phosphate to glucose to form glucose-6-phosphate. ... Suppose that a cell has only glucose available for energy and that the activity of hexokinase is suddenly stopped in this cell.

Does hexokinase need ATP?

Hexokinase (HK), a glycolytic, regulatory enzyme, phosphorylates glucose to glucose-6-phosphate. During this investment step, a phosphate is cleaved from adenosine triphosphate (ATP), providing a phosphate for glucose-6-phosphate via conversion of ATP to adenosine diphosphate (ADP).

How is hexokinase activated?

Hexokinase activates glycoloysis by phosphorylating glucose. ... Tissues where hexokinase is present use glucose at low blood serum levels. G6P inhibits hexokinase by binding to the N-terminal domain(this is simple feedback inhibition). It competitively inhibits the binding of ATP [8].