As you can see, there are more K+ and anions in the intracellular fluid, and more Na+ and Cl- in the extracellular fluid. While K+ is positively charged and more abundant on the inside, there exists a great amount of negatively charged particles (the anions), accounting for the negative charge inside the membrane.

Is intracellular more negative than extracellular?

There is a higher concentration of potassium ions on the inside of the cell than on the outside. ... That is, the inside of the cell becomes negative relative to the outside, setting up a difference in electrical potential across the membrane.

Is ECF positive or negative?

The ECF composition is therefore crucial for their normal functions, and is maintained by a number of homeostatic mechanisms involving negative feedback. Homeostasis regulates, among others, the pH, sodium, potassium, and calcium concentrations in the ECF.