Anthrone Reagent: Dissolve 200mg anthrone in 100mL of ice cold 95% H2SO4. Prepare fresh before use. Standard Glucose: Stock – Dissolve 100mg in 100mL water. Working standard – 10mL of stock diluted to 100mL with distilled water.
How do you make anthrone solution?
(i) Anthrone reagent: Dissolve 2g of Anthrone in 1 litre of concentrated H2SO4. Use freshly prepared reagent for the assay (ii) Glucose stock solution: 200μg glucose per mL distilled water. Note: Can include other carbohydrates of the same concentration if desired. boiling water bath for 10 minutes.
What is the anthrone method?
Anthrone test is a group test for carbohydrates that provides a rapid and convenient method for quantification of carbohydrates that are either free or bound to any lipids or proteins.
